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(KIA) Glucose fermentation & Peptone catabolized (K/K) No fermentation & Peptone catabolized Control (uninoculated) (KIA, H2S) Glucose fermentation & Peptone catabolized, Sulfur reduction If there is a black precipitate acid conditions must exist in the butt and we assume the butt would be yellow (A) if we could examine it BIOCHEMICAL TESTING PART TWO Results Continued: Next, solution C (zinc) is added to the tubes that showed no color change after addition of Nitrate A & B Zinc, a reducing agent, is capable of converting nitrate to nitrite; the development of a red color after addition of Nitrate C means that nitrates were present and reduced by the zinc not the organism; this is a negative result If the ...

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Urease test media contain 2% urea and phenol red as a pH indicator. An increase in pH due to the production of ammonia results in a color change from yellow (pH 6.8) to bright pink (pH 8.2). Urea broth (Stuart’s urea broth) is a highly buffered medium requiring large quantities of
Phenol Red or Pluronic™ Acid or Other Surfactants (1) Phenol red, sodium pyruvate (1) Phenol red, sodium pyruvate, L-glutamine (1) Pluronic™ Acid or other Surfactants (1) Ribonucleosides, Deoxyribosides and Sodium Bicarbonate (1) Sodium Bicarbonate (10) Sodium Bicarbonate and L-Glutamine (2) Sodium Bicarbonate, Glucose, L-Glutamine, Sodium ... Glucose Fermentation Test: Test to determine if the microbe can ferment the sugar glucose. The microbe is grown in a broth that contains glucose and the pH indicator, phenol red. If an organism is capable of fermenting the sugar glucose, then acidic byproducts are formed and the pH indicator turns yellow. Image obtained from

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Used to distinguish between enteric bacteria. In this test the bacteria are grown on Triple SUgar Iron agar. Has a peptone base and contains the pH indicator phenol red, which is yellow below 6.8. Also contains glucose (0.1%) sucrose and lactose (1%) and FeSO4
Feb 28, 1998 · Table 1 presents the test results of R. anatipestifer strains obtained by the conventional test procedure and the BSS test. Despite extensive retesting, the reference strain of R. anatipestifer did not show signs of acid production from carbohydrates in phenol red broth base but acidified glucose, fructose, mannose, maltose and dextrin in buffered carbohydrates. (ii) With addition of sugar and indicator (phenol red), may be used to study fermentation property of Neisseria. (c) Selective and Indicator Medium: (1) MacConkey’s agar (Fig. 10.12): Red, opaque solid medium in 45 mm radius petriplates, slants in test tubes. Composition and sterilisation. Uses:

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Phenol red is an acid-base indicator. Acid-base indicators are substances, which change colors depending upon the pH of the solution. Phenol red will change from red to yellow when the medium has a pH < 6.8 (more acidic) and from red to fuchsia when the medium has a pH >7.4 (less acidic). We will use two different kinds of phenol red broths. One contains glucose and one contains sucrose. If your bacterium . is. able to metabolize (eat) the sugar through the process of . fermentation
Phenol-Red Carbohydrate Broth with NaCl Grams/ Liter ... Phenol red 0.01 Final pH 6.8 ± 0.2at 25°C MICROBIOLOGICAL AGAR ... B. Methyl-red test Results: A. Phenol Red broth Record your results after incubation in the table Organism Results Interpretation color Gas production P. aeruginosa E. coli Glucose Lactose Glucose Lactose Glucose Lactose Glucose Lactose S. aureus P. vulgaris Question: 1.

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Phenol Red (PR) Broth is a differential test medium prepared as a base to which a carbohydrates is added. Included in the base medium are peptone and the pH indicator phenol red. We will inoculate PR Glucose Broths with three organisms to determine their fermentation characteristics. Q1. Suppose you innoculate a PR broth with a slow-growing ...
(KIA) Glucose fermentation & Peptone catabolized (K/K) No fermentation & Peptone catabolized Control (uninoculated) (KIA, H2S) Glucose fermentation & Peptone catabolized, Sulfur reduction If there is a black precipitate acid conditions must exist in the butt and we assume the butt would be yellow (A) if we could examine it Phenol red broth is a test that is differential for gram negative bacteria. It tests for three different things: the the ability of a microbe to ferment glucose, the ability to degrade amina acids, and the ability to produce gas as a byproduct of fermentation. The tube starts out orange colored.

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Methyl Red was the next biochemical test to be done, which the result was positive for glucose fermentation and mixed acids. The Urea test was the final test. The results were positive for urease, which eliminated all but Staphylococcus epidermidis as the Gram-positive unknown microorganism for the mixed culture of unknown 101. Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram negative enteric bacteria. It contains peptone, phenol red (a pH indicator), a Durham tube, and one carbohydrate (glucose, lactose, or sucrose).

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Results of these tests are compared to established taxa or identification schemes (Appendix 1.2). Examples of selective media are thiosulfate citrate bile sucrose agar (TCBS) for vibrios, and glutamate starch phenol red agar (GSP) for aeromonads and peudomonads.
The second part of the test was for lactose fermentation with my results coming back -, indicating that no fermentation occurred as a result of the red broth. The final part of the Phenol Red Broth tested for sucrose fermentation and my results were were + based on the yellow broth, which meant fermentation with acid had occurred.